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<t>(A)</t> <t>Time-lapse</t> imaging using a time-lapse camera. (B) Representative images captured by the time-lapse camera (red circles indicate the position of H. axyridis pupa). The bottom-left image shows the pupa. (C) Instruments used for dissection and staining. (D) Dissection procedure. (E) Well plate containing wing primordia during phenoloxidase (PO) activity staining.
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<t>(A)</t> <t>Time-lapse</t> imaging using a time-lapse camera. (B) Representative images captured by the time-lapse camera (red circles indicate the position of H. axyridis pupa). The bottom-left image shows the pupa. (C) Instruments used for dissection and staining. (D) Dissection procedure. (E) Well plate containing wing primordia during phenoloxidase (PO) activity staining.
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<t>(A)</t> <t>Time-lapse</t> imaging using a time-lapse camera. (B) Representative images captured by the time-lapse camera (red circles indicate the position of H. axyridis pupa). The bottom-left image shows the pupa. (C) Instruments used for dissection and staining. (D) Dissection procedure. (E) Well plate containing wing primordia during phenoloxidase (PO) activity staining.
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<t>(A)</t> <t>Time-lapse</t> imaging using a time-lapse camera. (B) Representative images captured by the time-lapse camera (red circles indicate the position of H. axyridis pupa). The bottom-left image shows the pupa. (C) Instruments used for dissection and staining. (D) Dissection procedure. (E) Well plate containing wing primordia during phenoloxidase (PO) activity staining.
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<t>(A)</t> <t>Time-lapse</t> imaging using a time-lapse camera. (B) Representative images captured by the time-lapse camera (red circles indicate the position of H. axyridis pupa). The bottom-left image shows the pupa. (C) Instruments used for dissection and staining. (D) Dissection procedure. (E) Well plate containing wing primordia during phenoloxidase (PO) activity staining.
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<t>(A)</t> <t>Time-lapse</t> imaging using a time-lapse camera. (B) Representative images captured by the time-lapse camera (red circles indicate the position of H. axyridis pupa). The bottom-left image shows the pupa. (C) Instruments used for dissection and staining. (D) Dissection procedure. (E) Well plate containing wing primordia during phenoloxidase (PO) activity staining.
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(A) Time-lapse imaging using a time-lapse camera. (B) Representative images captured by the time-lapse camera (red circles indicate the position of H. axyridis pupa). The bottom-left image shows the pupa. (C) Instruments used for dissection and staining. (D) Dissection procedure. (E) Well plate containing wing primordia during phenoloxidase (PO) activity staining.

Journal: Bio-protocol

Article Title: A Simple Method for Estimating the Spatiotemporal Distribution of Phenoloxidase Proteins in Insect Tissues

doi: 10.21769/BioProtoc.5646

Figure Lengend Snippet: (A) Time-lapse imaging using a time-lapse camera. (B) Representative images captured by the time-lapse camera (red circles indicate the position of H. axyridis pupa). The bottom-left image shows the pupa. (C) Instruments used for dissection and staining. (D) Dissection procedure. (E) Well plate containing wing primordia during phenoloxidase (PO) activity staining.

Article Snippet: Time-lapse camera (SANWA SUPPLY, model: 400-CAM109) 10.

Techniques: Imaging, Dissection, Staining, Activity Assay